Journal:

Article Title: ?' Fibrinogen: Evaluation of a New Assay for Study of Associations with Cardiovascular Disease

doi: 10.1373/clinchem.2009.138347

Figure Lengend Snippet: The analytical specificity of anti-γ' fibrinogen monoclonal antibody 2.G2.H9 and its ability to differentiate γ' fibrinogen from fibrinogen lacking γ' chains was determined by incubating the indicated dilutions of the antibody with γA/γA or γA/γ' fibrinogen in microtiter wells. Goat anti-mouse IgG/HRP conjugate was added for detection, followed by O-phenylenediamine, and the absorbance was quantitated at 450 nm.

Article Snippet: HRP-conjugated sheep anti-human fibrinogen (Innovative Research, Inc.) was diluted 1:2500 in PBS/0.1% BSA/0.1% Triton X-100, and 50 μl was added per well, incubating for 1 hour at 37°C.

Techniques:

Journal:

Article Title: ?' Fibrinogen: Evaluation of a New Assay for Study of Associations with Cardiovascular Disease

doi: 10.1373/clinchem.2009.138347

Figure Lengend Snippet: A γ' fibrinogen standard curve was generated using the indicated concentrations of purified γ' fibrinogen reconstituted in heat-defibrinated plasma. Capture antibody was anti-γ' fibrinogen monoclonal antibody 2.G2.H9, and detection antibody was a commercial sheep anti-human fibrinogen/HRP conjugate. TMB substrate absorbance was quantitated at 450 nm. The resulting points were fit either to a linear, logarithmic, or second-degree polynomial curve fit.

Article Snippet: HRP-conjugated sheep anti-human fibrinogen (Innovative Research, Inc.) was diluted 1:2500 in PBS/0.1% BSA/0.1% Triton X-100, and 50 μl was added per well, incubating for 1 hour at 37°C.

Techniques: Generated, Purification